Exclusion Chromatography Services

Biopharmaceutical and life science industries rely on the GL Technologies team of experts to help their organizations maintain FDA and cGMP compliance which can include calibration of manufacturing equipment, HPLC calibration, lot repeatability testing, and product safety testing.

Exclusion chromatography services are an important tool for separating molecules based on their size. This technique is widely used in the biopharmaceutical industry for purifying macromolecules such as proteins, DNA, and viruses.

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What is Exclusion Chromatography?

Exclusion chromatography is a separation technique that separates molecules based on their size. It works by passing a mixture of molecules through a column packed with a stationary phase that has pores of a specific size. The larger molecules cannot enter the pores and, therefore, flow around the particles and elute first, while the smaller molecules enter the pores and are retained longer.

How Does Exclusion Chromatography Work?

Exclusion chromatography services work by utilizing a gel filtration column, which contains beads or resins with pores of a specific size. The size of the pores can be adjusted by selecting a specific type of resin, such as Sephadex or Sepharose. The stationary phase is packed into a column, and the sample mixture is applied to the top of the column. The sample mixture then flows through the stationary phase, and the molecules are separated based on their size.

Exclusion Chromatography Lab Applications

Exclusion chromatography services have a wide range of applications in the biopharmaceutical industry, including:

Protein Purification

Exclusion chromatography is an amazing technique that is used for protein purification and separation based on size. It operates on the idea that molecules diffuse more easily through a porous matrix, while larger moleculres are excluded earlier. This is a useful method for separating proteins from a complex mixture because it allows the isolation of target proteins based on their molecular weight. Using a column packed with a gel matrix and defined pore site, proteins of different sizes will be able to be separated. Exlusion chromatography is a valuable tool for the purification and characterization of proteins.&

Virus Purification

Exclusion chromatography is based on the principle of size exclusion, where the stationary phase contains porous beads with specific pore sizes. When a virus sample is applied to the column, smaller molecules such as contaminants can enter the pores and get trapped, while larger viruses pass through unaffected, resulting in the separation from unwanted components. With this technique, researchers can isolate and purify viruses, while verifying the quality of viral preparations for various applications, including diagnostics, vaccine development, and fundamental virology research.

DNA Purification

In DNA purification, exclusion chromatography is used to separate and remove unwanted impurities such as proteins, RNA, and other contaminants from the DNA sample. The method involves passing the sample through a porous gel, where smaller molecules get trapped within the pores, while larger DNA molecules flow freely through the column, resulting in their purification. This technique has proven to be invaluable in various fields, including molecular biology, genetics, and biotechnology, allowing researchers to obtain highly pure DNA samples for downstream applications such as sequencing, cloning, and gene expression analysis.

Analytical Chromatography

Analytical chromatography includes a range of techniques such as gas chromatography and HPLC (high performance liquid chromatography), which are both used for quantitative analysis of compounds in a mixture. These chromatography techniques use a stationary phase and a mobile phase to separate and detect individual components based on their chemical properties such as polarity and size. They are widely used in the biopharmaceutical and life science industries during research and development.