Exclusion Chromatography Services
Biopharmaceutical and Medical Device industries rely on the GL Technologies team of experts to ensure FDA cGMP compliance, product efficacy, lot repeatability, safety of patients, utilities and manufacturing equipment repeatability, and safety of personnel. We provide full-service HPLC Calibration and HPLC certification for our clients of all sizes throughout the state of California.
Exclusion chromatography services are an essential tool for separating molecules based on their size. This technique is widely used in the biopharmaceutical industry for purifying macromolecules such as proteins, DNA, and viruses. In this article, we will discuss what exclusion chromatography services are, how they work, and their applications.
To speak with the experts about your Exclusion HPLC Calibration needs, please fill out our online form or give us a call!
What is Exclusion Chromatography?
Exclusion chromatography is a separation technique that separates molecules based on their size. It works by passing a mixture of molecules through a column packed with a stationary phase that has pores of a specific size. The larger molecules cannot enter the pores and, therefore, flow around the particles and elute first, while the smaller molecules enter the pores and are retained longer.
How Does Exclusion Chromatography Work?
Exclusion chromatography services work by utilizing a gel filtration column, which contains beads or resins with pores of a specific size. The size of the pores can be adjusted by selecting a specific type of resin, such as Sephadex or Sepharose. The stationary phase is packed into a column, and the sample mixture is applied to the top of the column. The sample mixture then flows through the stationary phase, and the molecules are separated based on their size.
Applications of Exclusion Chromatography
Exclusion chromatography services have a wide range of applications in the biopharmaceutical industry, including:
Protein Purification: Exclusion chromatography is an essential technique for purifying proteins from complex mixtures, such as cell lysates or fermentation broths.
Virus Purification: Exclusion chromatography is also used to purify viruses from complex mixtures, such as cell culture supernatants.
DNA Purification: Exclusion chromatography can be used to purify DNA fragments from PCR reactions or to remove contaminants from plasmid preparations.
Analytical Chromatography: Exclusion chromatography is also used for the analysis of macromolecules. It can be used to determine the molecular weight and size of a sample and to quantify the purity of a protein preparation.
To speak with the experts about your Exclusion HPLC Calibration needs, please fill out our online form or give us a call!
Exclusion chromatography services are an essential tool for separating molecules based on their size. This technique is widely used in the biopharmaceutical industry for purifying macromolecules such as proteins, DNA, and viruses. The ability to adjust the size of the pores of the stationary phase allows for the separation of a wide range of molecules. Exclusion chromatography is a powerful technique that is used extensively in biopharmaceutical research and development.