Hydrophobic Interaction HPLC Chromatography Services

The team at GL Technologies works with a variety of biopharmaceutical organizations both large and small for their HPLC calibration and HPLC certification laboratory requirements. We have worked hard to earn the trust of our clients and provide full-service HPLC calibration services according to their specific needs. Helping our clients maintain FDA and cGMP requirements for product safety, lot repeatability, and equipment calibrations. Hydrophobic interaction chromatography (HIC) is a powerful technique that has been widely used in the field of protein purification. It is based on the principle that nonpolar groups on the surface of a protein will interact with hydrophobic ligands on a chromatography resin in the presence of high salt concentrations. This interaction allows for selective separation of the target protein from other components in a mixture.

To speak with the experts about your Hydrophobic Interaction HPLC Calibration needs, please fill out our online form or give us a call!

858-202-1408

Basis of Hydrophobic Interaction HPLC Calibration

The basis of HIC lies in the fact that proteins have hydrophobic patches on their surface, which can interact with hydrophobic ligands on the surface of the chromatography resin. The hydrophobic patches on a protein are formed by the presence of nonpolar amino acids such as leucine, isoleucine, valine, phenylalanine, and tryptophan. These amino acids are typically found in the interior of a protein, but can also be exposed on the protein surface due to conformational changes or denaturation.

The chromatography resin used in HIC typically consists of hydrophobic ligands such as butyl, octyl, or phenyl groups attached to a solid support. The hydrophobic groups on the resin interact with the hydrophobic patches on the protein, resulting in the protein being bound to the resin. The strength of the interaction between the protein and the resin is influenced by the salt concentration of the buffer used in the chromatography process. At high salt concentrations, the hydrophobic interaction between the protein and the resin is weakened, allowing for elution of the protein from the column.

The Role of GL Technologies

Hydrophobic Interaction Chromatography is a complex and highly specialized technique that requires expertise and state-of-the-art equipment to yield accurate results. This is where GL Technologies can help.  We provide a range of services to support researchers and industries in their analytical and purification needs.

Customized Method Development

One of the primary services offered by GL Technologies is the development of customized HIC methods. Since HIC is often used for biomolecule purification, we tailor our methods to specific molecules or sample matrices. This verifies the best separation and purification results, saving time and resources.

Sample Analysis and Testing

We have advanced analytical instruments and experienced technicians who can analyze and test samples provided by clients. This service is especially valuable in research and quality control applications where precise characterization of compounds is essential.

Purification Services

For biopharmaceuticals, biotechnology, and biochemistry, the purification of biomolecules is a critical step. GL Technologies offers purification services using their specialized equipment and expertise. This helps in obtaining high-purity products efficiently.

Hydrophobic Process Involves Several Steps

Including equilibration of the column with a buffer containing high salt concentration, loading of the protein sample onto the column, washing with a buffer containing decreasing salt concentration to remove non-specifically bound impurities, and elution of the protein with a buffer containing low salt concentration. The eluted protein can be further purified by additional chromatography steps or other purification techniques.

One of the advantages of HIC is its compatibility with a wide range of buffer conditions, including high salt concentrations and denaturants such as urea or guanidine hydrochloride. This allows for purification of proteins that are difficult to purify using other chromatography techniques. HIC is also a relatively gentle purification technique, which can help to preserve the activity and stability of the target protein.

Hydrophobic interaction chromatography is a powerful technique for the purification of proteins based on their hydrophobic properties. It is a versatile and gentle purification technique that can be used to purify a wide range of proteins under different buffer conditions.

From drug development to protein purification, hydrophobic interaction chromatography remains a powerful technique. GL Technologies works hard for our clients in supporting their research by providing specialized services that facilitate accurate separation and purification processes. When choosing to work with GL Technologies, you are prioritizing expertise, equipment, and reputation so that your specific needs and requirements are met.

About GL Technologies

GL Technologies (formally Calibration Consultants) is a full service calibration provider specializing in the Biopharmaceutical and Medical Device industries. Our management has worked in, managed, and developed FDA cGMP fully compliant programs. Our services include roving crews for the Biopharmaceuticals for regular scheduled calibrations or shutdown and new system situations. GL Technologies also places qualified individuals on various large and small Biopharmaceutical and Medical Device companies. The services we provide include general Calibration/ Metrology support, asset and CMMS management, compliant program development, SOP development, and maintenance/ facilities support.

To speak with the experts about your HPLC Calibration needs, please fill out our online form or give us a call!

858-202-1408