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San Diego Dionex HPLC Calibration Services

High-performance liquid chromatography (HPLC) is an analytical technique that is widely used in laboratories to separate, identify, and quantify the components of a sample. Accurate and precise results from an HPLC analysis depend heavily on proper calibration of the instrument. Calibration is a critical process that ensures the accuracy and reliability of your HPLC system. We have the expertise to calibrate a Dionex HPLC system. The team of experts at GL Technologies works with a variety of biopharmaceutical and related industries throughout the San Diego region to calibrate their on-site Dionex HPLC equipment.

To speak with the experts about your Dionex HPLC Calibration needs, please fill out our online form or give us a call!


The Role of a Specialized HPLC Calibration Provider

While HPLC calibration can be performed in-house, many laboratories opt for GL Technologies to perform their HPLC calibrations due to numerous advantages:


The team at GL Technologies are highly trained technicians with extensive experience in calibration HPLC systems of all brands, including Dionex. We are well-versed in the intricacies of different HPLC models and can identify and fix issues quickly and efficiently.


In regulated industries like biopharmaceutical and pharmaceuticals, adherence to strict quality and compliance standards is required. GL Technologies makes sure our clients’ HPLC systems comply with industry standards, reducing the risk of compliance-related issues and audits.

Time and Cost Savings

Outsourcing calibration services to the professionals at GL Technologies saves you valuable time and money. Our HPLC technicians have the necessary tools and experience to complete the calibration quickly, minimizing downtime and costly disruptions to your laboratory operations.


Accurate record-keeping is vital in any laboratory setting. GL Technologies provides comprehensive calibration documentation, helping our clients maintain a clear audit trail and ensuring traceability of results.

Why Calibration is Important

HPLC is a highly sensitive technique that can detect very small changes in the sample. However, even a small error in the calibration of the system can lead to significant errors in the results. Calibration is the process of verifying and adjusting the performance of the HPLC system to ensure that it is operating within acceptable limits. Calibration is essential to ensure that the instrument is providing accurate and precise results, and that the data generated is reliable.

Calibration of a Dionex HPLC System

Calibration of a Dionex HPLC system involves several steps, including the following:

Check the Instrument Performance: Before beginning the calibration process, check the performance of the instrument. Check the mobile phase, column, and detector for proper functioning.

Check the System Pressure: The system pressure should be checked to ensure that it is within acceptable limits. An excessively high or low system pressure can lead to poor chromatographic performance.

Prime the HPLC System: Prime the HPLC system to ensure that there are no air bubbles in the lines, and that the system is free of leaks.

Prepare the Calibration Standards: Prepare the calibration standards according to the manufacturer's instructions. The standards should be prepared using high-quality reagents to ensure accuracy and precision.

Inject the Calibration Standards: Inject the calibration standards into the HPLC system, and record the peak areas and retention times for each standard.

Calculate the Calibration Curve: Use the data obtained from the calibration standards to calculate the calibration curve. The calibration curve is a plot of peak area versus concentration.

Verify the Calibration: Verify the calibration by injecting a sample and comparing the results to the calibration curve. The results should fall within the acceptable limits.

Document the Calibration: Document the calibration results, including the calibration curve and any adjustments made to the system.

About GL Technologies

GL Technologies (formally Calibration Consultants) is a full service calibration provider specializing in the Biopharmaceutical and Medical Device industries. Our management has worked in, managed, and developed FDA cGMP fully compliant programs. Our services include roving crews for the Biopharmaceuticals for regular scheduled calibrations or shutdown and new system situations. GL Technologies also places qualified individuals on various large and small Biopharmaceutical and Medical Device companies. The services we provide include general Calibration/ Metrology support, asset and CMMS management, compliant program development, SOP development, and maintenance/ facilities support.

To speak with the experts about your Dionex HPLC Calibration needs, please fill out our online form or give us a call!



To request a quote from GL-Tec, please fill out our online form and we will get back to you promptly.


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