San Diego Dionex HPLC Calibration Services
High-performance liquid chromatography (HPLC) is an analytical technique that is widely used in laboratories to separate, identify, and quantify the components of a sample. Accurate and precise results from an HPLC analysis depend heavily on proper calibration of the instrument. Calibration is a critical process that ensures the accuracy and reliability of your HPLC system. We have the expertise to calibrate a Dionex HPLC system. The team of experts at GL Technologies works with a variety of biopharmaceutical and related industries throughout the San Diego region to calibrate their on-site Dionex HPLC equipment.
To speak with the experts about your Dionex HPLC Calibration needs, please fill out our online form or give us a call!
Why Dionex Calibration is Important
HPLC is a highly sensitive technique that can detect very small changes in the sample. However, even a small error in the calibration of the system can lead to significant errors in the results. Calibration is the process of verifying and adjusting the performance of the HPLC system to ensure that it is operating within acceptable limits. Calibration is essential to ensure that the instrument is providing accurate and precise results, and that the data generated is reliable.
Calibration of a Dionex HPLC System
Calibration of a Dionex HPLC system involves several steps, including the following:
Check the Instrument Performance: Before beginning the calibration process, check the performance of the instrument. Check the mobile phase, column, and detector for proper functioning.
Check the System Pressure: The system pressure should be checked to ensure that it is within acceptable limits. An excessively high or low system pressure can lead to poor chromatographic performance.
Prime the HPLC System: Prime the HPLC system to ensure that there are no air bubbles in the lines, and that the system is free of leaks.
Prepare the Calibration Standards: Prepare the calibration standards according to the manufacturer's instructions. The standards should be prepared using high-quality reagents to ensure accuracy and precision.
Inject the Calibration Standards: Inject the calibration standards into the HPLC system, and record the peak areas and retention times for each standard.
Calculate the Calibration Curve: Use the data obtained from the calibration standards to calculate the calibration curve. The calibration curve is a plot of peak area versus concentration.
Verify the Calibration: Verify the calibration by injecting a sample and comparing the results to the calibration curve. The results should fall within the acceptable limits.
Document the Calibration: Document the calibration results, including the calibration curve and any adjustments made to the system.
To speak with the experts about your Dionex HPLC Calibration needs, please fill out our online form or give us a call!
Calibration of a Dionex HPLC system is a critical process that ensures the accuracy and reliability of your HPLC analysis. It is important to follow the manufacturer's instructions carefully and use high-quality reagents to prepare the calibration standards. By properly calibrating your HPLC system, you can be confident that your results are accurate and precise, and that the data generated is reliable.