Service/Sales/ General Inquiries 858-202-1408

Los Angeles HPLC Agilent Calibration

As Los Angeles expert providers of Agilent HPLC calibration and certification services, GL Technologies creates a customized performance protocl specifically for your instrument/s. Our team provides testing with NIST traceable test equipment to maintain scientific accuracy. Once our testing is complete, we will outline the performance characteristics of the instruments that were calibrated.  This will in turn provide important information about the instrument operation and meet compliance requirements with the outside regulatory agencies, and in-house QA programs.

To speak with the experts about your HPLC calibration needs, please fill out our online form or give us a call!


HPLC Calibration Consultants

The testing programs GL Technologies implements will stand up against any external (or internal) regulatory scrutiny.

With the most experienced Los Angeles HPLC consultants in the industry, we've performed 100s of HPLC calibration performance tests for organizations of all sizes in the pharmaceutical, biomedical and bio-pharmaceutical industries. Your HPLC equipment will be thoroughly tested and calibrated and we can gladly supply references upon request.

Affordable HPLC Calibration

GL Technologies provides competitive prices that are often 30% lower than what the OEM services department charges, with NO reduction in quality. You simply provide us the instrument (either on-site or delivered), and we take it from there. All the performance specifications for your HPLC instrument will be tested and formatted for ISO 17025 certification.

We don't just do Los Angeles HPLC calibration and performance testing, we also will provide your lab with a complete Preventative Maintenance program and HPLC instrument repairs. Those 2 together, we will ensure that the instruments in your laboratory are within specifications and maintained at the optimum state of reliability.

What is Agilent HPLC Calibration?

Agilent HPLC calibration refers to the systematic process of making sure the accuracy and precision of measurements taken by High-Performance Liquid Chromatography (HPLC) systems manufactured by Agilent Technologies. Calibration involves a series of standardized procedures that verify and adjust the instrument's settings, such as flow rate, detector sensitivity, and injection volume, to match with established reference standards. This calibration process is important for obtaining reliable and reproducible results in HPLC analysis. It verifies that the instrument operates within specified parameters, guaranteeing the accuracy of compound identification and quantification in a sample, maintaining the integrity and reliability of analytical data in various scientific and industrial applications.

Which HPLC Components Need Calibration?

Mobile phase – acts as a carrier stream for the sample
Injector – injects fixed volume of sample into the mobile phase stream
Pump – delivers mobile phase at constant flow rate
Column– separates the sample components
Column oven – maintains constant column temperature
Detector – detects individual eluting sample components

Key Calibration Benefits

Calibrating an Agilent high-performance liquid chromatography (HPLC) system is important for maintaining accuracy, precision, and reliability in analytical results. Here are 3 benefits of performing Agilent HPLC calibration:

Accuracy and Precision

Calibration helps in fine-tuning and adjusting the system to maintain accurate and precise measurements. By calibrating various parameters including flow rate, pressure, detector sensitivity, and retention times, the HPLC system can deliver accurate and reliable results. This accuracy is important for analytical procedures where even the smallest variations can significantly impact the outcome.

Peak Performance

Regular calibration of an Agilent HPLC system maintains peak performance. It helps identify and fix any deviations or inaccuracies in the system. This process makes sure that the instrument functions at its peak efficiency, increasing productivity by minimizing downtime due to potential issues that may arise from an uncalibrated system.

Compliance with Regulations

In various industries such as pharmaceuticals, environmental analysis, food safety, and more, adherence to regulatory standards is imperative. Calibration of the Agilent HPLC system validates that the instrument complies with industry standards and regulatory requirements. Regular calibration helps in meeting validation criteria and the reliability and traceability of the analytical results, which is vital for quality control and meeting regulatory obligations.

Steps for Calibrating an Agilent HPLC System


The preparation phase involves several key steps to guarantee a successful calibration process. To start, the GL-Tec team thoroughly cleans the system components, including the solvent lines, pump, injector, and detector, using a ssolvent to eliminate any residue or contaminants that might affect the calibration. We then prepare the mobile phase by accurately measuring and mixing the appropriate solvents according to the analytical method requirements, maintaining proper filtration to remove particulates. After this, we equilibrate the system by running the mobile phase through all components to stabilize the system temperature and pressure, allowing for consistent performance during calibration.

System Suitability Test

GL-Tec prepares the mobile phase by accurately measuring and mixing the solvent components following the protocol specified in the method. We then equilibrate the system by running the mobile phase through the HPLC columns at the recommended flow rate and temperature for a sufficient duration to stabilize the system. Then, inject a standard solution containing known compounds into the system to evaluate key parameters including retention time, peak symmetry, resolution, and tailing factor. Analyze the chromatogram to confirm that these parameters meet the acceptance criteria outlined in the method. Our team then makes necessary adjustments to the system settings if any parameter falls outside the specified limits. Re-run the system suitability test until all parameters meet the criteria, making sure that the HPLC system is accurately calibrated for subsequent analyses.

Flow Rate Calibration

This calibration process involves determining the actual flow rate of the mobile phase through the system, comparing it with the set or designated flow rate, and making necessary adjustments to achieve the desired rate. It begins by priming the system to remove any air bubbles and stabilize the flow, followed by using a calibrated flowmeter or software-integrated flow rate detectors to measure the actual flow. Any deviations observed are then corrected by adjusting the pump settings or system parameters until the measured flow aligns precisely with the target flow rate

Injector Calibration

Injector calibration involves fine-tuning the injector settings to guarantee consistent and reproducible delivery of samples into the chromatographic column. It involves adjustments to parameters including injection volume, injection speed, and wash cycles, tailored to specific sample characteristics and analytical requirements. Accurate injector calibration not only increases the reliability of analytical results but also minimizes carryover effects, which maintains the system's efficiency and data integrity in analysis.

Column Efficiency and Retention Time Calibration

Column efficiency, ocharacterized by the theoretical plates, is the separation capability of the chromatographic column. A higher number of theoretical plates reflects better resolution and separation of analytes within the sample. Calibration of retention time maintains precise identification and quantification of compounds by establishing a correlation between the retention times and the compounds being analyzed. This calibration process involves the use of standard compounds with known retention times, aiding in the accurate determination of unknown substances present in the sample.

Detector Calibration

The detector, an important component of the Agilent HPLC setup, requires periodic calibration to validate its response and sensitivity to various analytes. Calibration involves a series of steps, using standard reference compounds at known concentrations to create a calibration curve. This curve aids in establishing the relationship between the detector's signal output and the concentration of the analyte, allowing for the accurate quantification during sample analysis. Regular detector calibration not only guarantees the reliability of analytical results but also maintains the instrument's performance, creating consistency and credibility in analysis across various applications in pharmaceuticals and biopharmaceuticals.

Documentation and Record-Keeping

Accurate and detailed records should include system maintenance, calibration, and method development for making sure the reproducibility and traceability of results are accurate. A well-organized documentation process includes all facets such as instrument parameters, column details, mobile phase composition, and standard operating procedures (SOPs). Regular maintenance logs, detailing system checks, repairs, and component replacements, are vital for instrument performance. Documentation also maintains compliance with regulatory standards.

HPLC Articles

Fine-tuning HPLC Calibration for Biopharma Excellence

In the evolving world of biopharmaceuticals, High-Performance Liquid Chromatography (HPLC) stands as a pillar analytical technique. It allows for the separation, identification, and quantification of compounds that are vital to the development, production, and quality control of biopharmaceuticals. HPLC calibration plays a role in maintaining the accuracy, reliability, and excellence of analytical results in this industry.

The Intersection of HPLC and Biopharmaceutical Innovation

In the wordl of biopharmaceuticals, the advancement of analytical techniques plays a pivotal role in making sure the safety, efficacy, and quality of therapeutic drugs. High-Performance Liquid Chromatography (HPLC) stands at the forefront of these technological advancements, driving innovation and precision in the development and manufacturing processes of biopharmaceuticals.

Quality and Compliance: HPLC OQ in the Biopharmaceutical Industry

The biopharmaceutical industry plays an important role in developing and producing life-saving drugs and therapies. Maintaining the quality and safety of these products is of utmost importance. HPLC (high-performance liquid chromatography) is a widely used analytical technique in the biopharma industry for assessing the quality and purity of drugs and biologics. To meet regulatory standards for quality, organizations perform HPLC Operational Qualification (OQ). Let’s jump into the significance of HPLC OQ in the biopharma industry, its processes, and the role it plays in meeting compliance and product integrity.

About GL Technologies

As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!



To request a quote from GL-Tec, please fill out our online form and we will get back to you promptly.


GL Technologies Location