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Los Angeles HPLC Equipment Calibration Company

The team at GL Technologies provides HPLC equipment calibration services to biopharmaceutical and related industries throughout the Los Angeles area.  High-performance liquid chromatography (HPLC) is a widely used analytical technique for separating and analyzing complex mixtures of chemicals. To ensure accurate and reliable results, it is important to calibrate the HPLC equipment regularly. Calibration is the process of verifying and adjusting the performance of the HPLC equipment to meet predefined specifications. In this blog, we will discuss the steps involved in HPLC equipment calibration.  Our team is ready to help you with all your HPLC calibration needs!

To speak with the experts about your HPLC Calibration needs, please fill out our online form or give us a call!

858-202-1408

GL-Tec HPLC Calibration Services


The GL-Tec team of experts provides every step in the Los Angeles HPLC equipment calibration process includes:

Step 1: Preparation


Before starting the calibration process, ensure that the HPLC equipment is clean and free from any residue. Remove any previous sample residues from the HPLC column by flushing with the mobile phase. Ensure that the HPLC column is properly installed and the mobile phase is prepared as per the manufacturer's instructions.

Step 2: Check System Pressure


The first step in HPLC equipment calibration is to check the system pressure. This can be done by running the HPLC system at the specified flow rate and measuring the system pressure using a pressure gauge. Compare the measured pressure with the recommended pressure range specified by the manufacturer. If the measured pressure is outside the recommended range, investigate the cause and take corrective action.

Step 3: Verify Flow Rate


Next, verify the flow rate of the mobile phase. This can be done by using a flow meter or by weighing the mobile phase before and after a fixed time interval. The measured flow rate should be within the specified range mentioned by the manufacturer.

Step 4: Detector Calibration


The detector calibration is an important step in HPLC equipment calibration. To calibrate the detector, inject a standard solution of a known concentration and measure the response of the detector. Calculate the detector response factor by dividing the peak area by the concentration of the standard solution. The response factor should be within the acceptable range specified by the manufacturer.

Step 5: Column Calibration


Column calibration is another crucial step in HPLC equipment calibration. It involves running a standard solution through the HPLC column and measuring the retention time of the analyte peak. Compare the retention time of the analyte peak with the reference standard retention time. If the retention time is within the acceptable range, the column is calibrated.

Step 6: System Suitability Test


The final step in HPLC equipment calibration is the system suitability test. This test ensures that the HPLC system is working within the acceptable limits. The test involves running a known sample through the HPLC system and measuring various parameters such as resolution, peak symmetry, and column efficiency. The measured parameters should be within the acceptable range specified by the manufacturer.

In conclusion, regular calibration of HPLC equipment is essential to ensure accurate and reliable results. The calibration process involves verifying the system pressure, flow rate, detector response, column performance, and system suitability. By following the above steps, you can ensure that your HPLC equipment is calibrated to meet the predefined specifications, and obtain accurate and reliable results.

To speak with the experts about your HPLC Calibration needs, please fill out our online form or give us a call!

858-202-1408

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