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San Diego Reverse HPLC Chromatography Biopharmaceutical Certification

Conveniently located in San Diego, GL Technologies has been serving the entire biopharmaceutical and related industries throughout San Diego for over 2 decades. Biopharmaceutical industries of all sizes need an expert team of HPLC specialists that can provide calibration and certification services to their organization's laboratories. When you need a trusted HPLC team, GL Technologies is here to help. Reverse HPLC chromatography is a specialized type of HPLC used for the purification and characterization of proteins and peptides.

To speak with the experts about your Reverse HPLC Calibration needs, please fill out our online form or give us a call!


Reverse HPLC Chromatography Services

Reverse HPLC chromatography is a technique used to separate proteins and peptides based on their hydrophobicity. In reverse HPLC, the stationary phase is a hydrophobic material, while the mobile phase is an aqueous buffer containing organic solvent. The hydrophobic stationary phase interacts with the hydrophobic regions of the protein or peptide, causing it to be retained longer in the column. The more hydrophobic the protein or peptide, the longer it will be retained in the column.

How is it Used in Research?

Reverse HPLC chromatography is an important tool used for a variety of reasons including:

Purity Assessment

Reverse HPLC is a technique that is widely used in the field of analytical chemistry for the assessment of "purity" in various substances. A stationary phase with different properties than the mobile phase, this method allows for the separation and identification of impurities within a sample. With exact measurements of retention times and peak areas, reverse HPLC allows for the quantification of impurities which provides valuable information for the quality of a compound.

Identification of Impurities

Reverse HPLC is used to identify and separate impurties in various samples, by using a reversed stationary phase and a mobile phase of appropriate polarity, this method allows for the separation and analysis of impurities in complex mixtures. Reverse HPLC chromatography offers selectivity and sensitivity, making it a popular tool in pharmaceutical research environments.


With reverse HPLC, the mobile phase is typically nonpolar, w hile the stationary phase is polar, providing separation of molecules based on their hydrophobic properties. Reverse HPLC chromatography provides valuable information about chemical composition and structural properties of samples. Researchers and laboratory workers can obtain valuable insights in the complex nature of compounds and make the right decisions regarding their properties and uses.

Batch-to-Batch Consistency

An important aspect of reverse HPLC is reaching batch-to-batch consistency, providing reliablity in the analytical results. With proper quality control measures, which includes equipment calibration and standardized sample preparation processes, reverse HPLC can consistently deliver exact outcomes across multiple batches. This creates reliability of the data and produces confident decision making, while maintaining product quality standards.

To speak with the experts about your Reverse HPLC Calibration needs, please fill out our online form or give us a call!



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