San Diego Reverse HPLC Chromatography Biopharmaceutical Certification
Conveniently located in San Diego, GL Technologies has been serving the entire biopharmaceutical and related industries throughout San Diego for over 2 decades. Biopharmaceutical industries of all sizes need an expert team of HPLC specialists that can provide calibration and certification services to their organization's laboratories. When you need a trusted HPLC team, GL Technologies is here to help.
High-performance liquid chromatography (HPLC) is a technique used to separate, identify, and quantify the components in a mixture. In biopharmaceutical manufacturing, HPLC is a critical tool for ensuring the safety and efficacy of drugs. Reverse HPLC chromatography is a specialized type of HPLC used for the purification and characterization of proteins and peptides. This article will discuss the importance of reverse HPLC chromatography in biopharmaceutical certification.
To speak with the experts about your Reverse HPLC Calibration needs, please fill out our online form or give us a call!
We Provide Reverse HPLC Chromatography Services
Reverse HPLC chromatography is a technique used to separate proteins and peptides based on their hydrophobicity. In reverse HPLC, the stationary phase is a hydrophobic material, while the mobile phase is an aqueous buffer containing organic solvent. The hydrophobic stationary phase interacts with the hydrophobic regions of the protein or peptide, causing it to be retained longer in the column. The more hydrophobic the protein or peptide, the longer it will be retained in the column.
Reverse HPLC Chromatography Important for Biopharmaceutical Certification?
Reverse HPLC chromatography is a critical tool for biopharmaceutical certification for several reasons:
Purity Assessment: Reverse HPLC chromatography is used to assess the purity of a protein or peptide. By separating the protein or peptide from impurities, reverse HPLC chromatography can provide an accurate assessment of the purity of the sample.
Identification of Impurities: Reverse HPLC chromatography can also be used to identify impurities in a sample. By analyzing the retention time of the impurities in the column, the nature of the impurities can be identified.
Characterization: Reverse HPLC chromatography can also be used to characterize the protein or peptide. By analyzing the retention time and elution profile of the protein or peptide, important characteristics such as molecular weight and conformational stability can be determined.
Batch-to-Batch Consistency: Reverse HPLC chromatography can be used to ensure batch-to-batch consistency in biopharmaceutical manufacturing. By analyzing the retention time and elution profile of the protein or peptide, it is possible to compare different batches and ensure that they are consistent in terms of purity and characteristics.
To speak with the experts about your Reverse HPLC Calibration needs, please fill out our online form or give us a call!
Reverse HPLC chromatography is an important tool for biopharmaceutical certification. It is used to assess the purity of a protein or peptide, identify impurities, and characterize the protein or peptide. Additionally, reverse HPLC chromatography can be used to ensure batch-to-batch consistency in biopharmaceutical manufacturing. By using reverse HPLC chromatography, biopharmaceutical companies can ensure the safety and efficacy of their products and maintain compliance with regulatory requirements.