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San Diego Reverse HPLC Certification

Conveniently located in San Diego, GL Technologies has been serving the entire biopharmaceutical and related industries throughout San Diego for over 2 decades. Biopharmaceutical industries of all sizes need an expert team of HPLC specialists that can provide calibration and certification services to their organization's laboratories. When you need a trusted HPLC team, GL Technologies is here to help. Reverse HPLC chromatography is a specialized type of HPLC used for the purification and characterization of proteins and peptides.

To speak with the experts about your Reverse HPLC Calibration needs, please fill out our online form or give us a call!


Reverse HPLC Chromatography Services

Reverse HPLC chromatography is a technique used to separate proteins and peptides based on their hydrophobicity. In reverse HPLC, the stationary phase is a hydrophobic material, while the mobile phase is an aqueous buffer containing organic solvent. The hydrophobic stationary phase interacts with the hydrophobic regions of the protein or peptide, causing it to be retained longer in the column. The more hydrophobic the protein or peptide, the longer it will be retained in the column.

How is it Used in Research?

Reverse HPLC chromatography is an important tool used for a variety of reasons including:

Purity Assessment

Reverse HPLC is a technique that is widely used in the field of analytical chemistry for the assessment of "purity" in various substances. A stationary phase with different properties than the mobile phase, this method allows for the separation and identification of impurities within a sample. With exact measurements of retention times and peak areas, reverse HPLC allows for the quantification of impurities which provides valuable information for the quality of a compound.

Identification of Impurities

Reverse HPLC is used to identify and separate impurties in various samples, by using a reversed stationary phase and a mobile phase of appropriate polarity, this method allows for the separation and analysis of impurities in complex mixtures. Reverse HPLC chromatography offers selectivity and sensitivity, making it a popular tool in pharmaceutical research environments.


With reverse HPLC, the mobile phase is typically nonpolar, w hile the stationary phase is polar, providing separation of molecules based on their hydrophobic properties. Reverse HPLC chromatography provides valuable information about chemical composition and structural properties of samples. Researchers and laboratory workers can obtain valuable insights in the complex nature of compounds and make the right decisions regarding their properties and uses.

Batch-to-Batch Consistency

An important aspect of reverse HPLC is reaching batch-to-batch consistency, providing reliablity in the analytical results. With proper quality control measures, which includes equipment calibration and standardized sample preparation processes, reverse HPLC can consistently deliver exact outcomes across multiple batches. This creates reliability of the data and produces confident decision making, while maintaining product quality standards.

Importance of Certification

Accuracy and Reliability

Certified reverse HPLC systems guarantee accurate and reproducible results, crucial for analytical laboratories. Through strict testing processes, certification verifies that the equipment performs consistently, minimizing errors and having reliable data output.


In highly regulated industries such as pharmaceuticals, adherence to industry standards and regulatory requirements is paramount. Certified reverse HPLC systems ensure compliance with regulations such as Good Laboratory Practices (GLP) and Good Manufacturing Practices (GMP), facilitating smooth audits and regulatory approvals.

Increased Efficiency

Certification involves calibration and validation procedures that optimize the performance of the equipment. Well-maintained and certified HPLC systems operate efficiently, reducing downtime and enhancing laboratory productivity.

Quality Assurance

Laboratories dealing with critical analyses rely on certified equipment to maintain quality assurance. Certification protocols involve routine maintenance, performance checks, and documentation, contributing to the overall quality management system of the laboratory.

Certification Process

The certification of reverse HPLC systems typically involves key steps IQ OQ PQ:

Installation Qualification (IQ)

Certificaiton starts with Installation Qualification (IQ), a step that validates that the system is properly installed and meets predefined specifications. IQ involves an assessment of the hardware and software components to verify their installation as per manufacturer's guidelines. This phase involves inspecting the instrument's physical installation, confirming proper connections, evaluating the functionality of individual components, and validating the software configuration. Testing is conducted to verify that the system operates according to predefined parameters and meets the specified standards for accuracy, precision, and performance

Operational Qualification (OQ)

Operational Qualification involves a series of steps to verify and validate the functionality and performance of the HPLC system in reverse mode. This certification process is a critical component within analytical chemistry, making sure that the equipment's compliance with predefined operational requirements and industry standards. It includes a variety of testing protocols, calibration checks, and systematic assessments of system suitability, column efficiency, flow rates, and detector sensitivity.

Performance Qualification (PQ)

The PQ phase validates the effectiveness of the HPLC system in reverse mode by subjecting it to strict tests and criteria. Performance Qualification is a series of assessments, including system suitability tests, precision, accuracy, and linearity evaluations, all with the goal of confirming the consistency and reliability of the HPLC system's performance.

About GL Technologies

As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.

To speak with the experts about your Reverse HPLC Calibration needs, please fill out our online form or give us a call!



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