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San Francisco Agilent HPLC Calibration Services

GL Technologies has become the premier provider for San Francisco pharmaceutical, biomedical and research laboratories for Agilent HPLC equipment calibration and certifications. Our team of experts has over 4 decades of experience in providing equipment calibrations for laboratories to ensure research efficiency and accuracy. We understand the pressure to produce accurate results is immense in the laboratory setting, if there are delays they can be costly. No matter if you are waiting for Agilent HPLC equipment repair or calibration, GL Technologies can help. We provide a new level of support through our preventative maintenance plans to deliver quality results, consistently.

Get faster response, faster access to Agilent HPLC experts, accurate results, and faster resolutions with GL-Tec

To speak with the experts about your Agilent HPLC needs, please fill out our online form or give us a call!


Understanding HPLC Calibration

HPLC calibration is the process of adjusting and validating the performance of an HPLC system to guarantee accurate and reproducible results. The calibration process involves a series of measurements and adjustments to various components including the pump, injector, column, and detector. In the world of biopharmaceuticals, where the smallest of variations can have significant implications for safety and effectiveness, calibration becomes even more important. It's imperative to maintain strict adherence to regulatory standards and industry best practices during calibration processes.

HPLC Modules that Require Regular Verification


The pump is a precisely designed component made of up several parts to deliver consistent flow rates. But, with regular usage and tear results in flow rate deviations its important that you have quick replacement of worn out parts to improve teh precision flow delivery. Regular calibration is still necessary. GL-Tecs pump calibration will establish flow rate accuracy and gradient composition accuracy.

Sample Injectors

A manual injection requires filling a fixed volume loop with the sample. In order to work properly the loop needs to be flushed continuously with the mobile phase so that th earlier sample traces are eliminated. Auto injectors are created to deliver precise volumes each time with sufficient wash cycles to ensure clean injections. When our team of experts does an injector calibration, it will provide confirmation of injector accuracy and linearity.

Column Oven

Retention times of compounds depend on mobile phase viscosity which is temperature dependent. The column needs to be maintained within specific temperature limits to get consistency. It is important to calibrate the temperature reproducibility of the column.


Detectors give absorbance signals that are dependent on wavelength of absorption of sample components. These signals can drop due to residual impurities and lamp life. The detector requires calibration regularly using compounds that are injected in to the mobile phase stream. The 2 main parameters that need calibration are linearity of response and wavelength accuracy.

HPLC is a very popular technique and several various makes are available. Laboratories have to adopt manufacturer prescribed calibration procedures at recommended intervals to obtain the desired accuracy and precise results.

GL-Tec measures every specification, every option, every time.

HPLC Calibration Provider

GL-Tech is a one-stop shop for all Agilent HPLC calibration services and certifications. Our calibration services are the convenient solution sto ensure on-going accuracy and availability of all the testing assets. Our team will work with your laboratory crew to create a customized solution that will include elecronic instruments as well as dimensional, physical, optical, and mechanical equipment!

Benefits of Agilent HPLC Calibration

Increased Accuracy and Precision

HPLC calibration plays an instrumental role in validating the accuracy and precision of analytical results. By calibrating the instrument, scientists and researchers can establish a correlation between the detected signals and the concentration of target compounds. This precision helps in quantifying substances within a sample with accuracy, which increases the credibility of analytical findings. Consistent calibration minimizes errors, maintaining reliable comparisons between different samples and experiments.

Quality Assurance and Compliance

Calibration is an indispensable component in providing quality control and meeting regulatory compliance standards in various industries. In biopharmaceutical and pharmaceuticals, adherence to strict quality standards is imperative. Proper HPLC calibration guarantees that the instrument operates within defined parameters, meeting industry regulations and validating the accuracy of results. It assures stakeholders, regulators, and consumers about the reliability of the data generated.

Instrument Performance

Regular calibration not only verifies accuracy but also improves the performance of HPLC instruments. Over time, components within the system may undergo wear or drift, impacting the instrument's accuracy. Calibration procedures help in detecting and fixing such deviations, which maintains the instrument's best functionality. This proactive approach not only extends the lifespan of the equipment but also minimizes the chances of instrument downtime, maintaining consistent productivity.

Reduction of Experimental Variability

Calibration also helps in reducing experimental variability, leading to more reproducible and consistent results. By establishing reliable baseline measurements through calibration, researchers can minimize fluctuations caused by environmental factors or minor instrument changes. Consistent calibration protocols allow for better control over experimental variables, allowing scientists to focus on the actual variations within samples, increasing the strength of scientific conclusions.

Cost-Efficiency and Time Savings

Investing time and resources in HPLC calibration may seem like an additional task, but it ultimately saves both time and money in the long run. Accurate calibration reduces the need for repeated analysis due to inaccurate results, saving resources and valuable time. Proactive maintenance and calibration prevent potential instrument failures or errors, avoiding costly downtime and repairs.

Popular HPLC Articles

HPLC Calibration: Accuracy in Analytical Chemistry

Analytical chemistry plays an important role in various scientific fields, from pharmaceuticals to environmental monitoring, where precision and accuracy are paramount. High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, allowing for the separation, identification, and quantification of components in complex mixtures. The reliability of HPLC results heavily relies on the proper calibration of equipment and attention to detail. Calibration is a process that verifies the accuracy, precision, and reliability of the HPLC system, protecting against errors that could compromise data integrity. Let's look at the significance of HPLC calibration, its key components, and the methods used to maintain accuracy in analytical chemistry.

Fine-tuning HPLC Calibration for Biopharma Excellence

In the evolving world of biopharmaceuticals, High-Performance Liquid Chromatography (HPLC) stands as a pillar analytical technique. It allows for the separation, identification, and quantification of compounds that are vital to the development, production, and quality control of biopharmaceuticals. HPLC calibration plays a role in maintaining the accuracy, reliability, and excellence of analytical results in this industry.

About GL Technologies

As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.

To speak with the experts about your Agilent HPLC needs, please fill out our online form or give us a call!



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