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San Francisco Shimadzu HPLC Calibration Services

Biopharmaceutical and medical laboratories throughout the San Francisco area that use Shimadzu equipment in their labs rely on the team at GL Technologies to provide top-quality calibration services for Shimadzu High-Performance Liquid Chromatography (HPLC) systems to ensure accurate and reliable results. Shimadzu HPLC systems are widely used in laboratories for various analytical applications such as pharmaceutical, environmental, and food testing. These systems consist of various components, including pumps, detectors, and columns, which need to be calibrated regularly to maintain their accuracy.

To speak with the experts about your Shimadzu HPLC needs, please fill out our online form or give us a call!


Calibration Services Include

At GL Technologies, we offer comprehensive Shimadzu HPLC calibration services to so that your HPLC system is functioning accurately.

Flow Rate Calibration

Maintaining an accurate flow rate maintains proper separation, elution, and detection of compounds within a chromatographic system. Key parameters in flow rate calibration involve the precise measurement and adjustment of the solvent flow rate using a flow meter or a specialized calibration device. Calibration protocols include verifying and fine-tuning the flow rate settings to match the intended specifications, which are vital for obtaining reproducible and reliable chromatographic results.

Detector Calibration

Sensitivity determines the detector's ability to detect and quantify low concentrations of analytes, often measured by the limit of detection (LOD) and limit of quantitation (LOQ). Linearity assesses the instrument's response across a range of concentrations, confirming its ability to produce a linear relationship between analyte concentration and detector response. Accuracy refers to the closeness of measured values to the true values, while precision assesses the instrument's reproducibility in delivering consistent results under similar conditions.

Column Calibration

Efficiency signifies the column's ability to separate compounds effectively, often gauged by the number of theoretical plates. Selectivity refers to the column's capability to differentiate between closely related compounds, highlighting its specificity. Resolution, the measure of peak separation, relies on the column's selectivity and efficiency. The calibration process involves improving these parameters through adjustment of mobile phase composition, flow rate, and column temperature.

Injection Volume Calibration

This parameter refers to the amount of sample introduced into the chromatographic system and significantly influences peak resolution, sensitivity, and system suitability. Injection volume provides accuracy and reproducibility of results, preventing errors in quantification and peak identification. Variations in injection volume can lead to shifts in retention times and peak shapes, affecting the reliability and accuracy of the analysis.

Why is Calibration Important?

Calibration is important so that the HPLC system is providing accurate and reliable results. Regular calibration helps to identify any deviations from the expected values and correct them before they impact your analysis. It is recommended to calibrate your HPLC system at least once a year or more frequently if it is used heavily.

Shimadzu HPLC Calibration Benefits

Increased Accuracy

Calibration by Shimadzu instruments fine-tunes parameters including flow rates, detector sensitivity, and retention times. These adjustments for your HPLC systems improve accuracy so that analytical results are dependable and precise. Researchers and analysts rely on this precision for confident decision-making in pharmaceutical, environmental, and food industries where variations matter.

Better Reproducibility

Staying 100% consistent in HPLC analysis is the key for reproducible results. GL Technologies Shimadzu's calibration procedures minimizes deviations, allowing for higher levels of reproducibility in experiments or sample analysis. This consistency is important for meeting regulatory requirements for compliance, creating trust in the generated data.

Extended Instrument Lifespan

Regular calibration by the GL Technologies experts in Shimadzu HPLC instruments will make sure instruments operate within the required ranges, reducing wear and tear while maintaining the performance required. This forward-thinking approach to maintenance can extend the lifespan of equipment, reducing the need for costly repairs or premature replacements.

Compliance with Industry Standards

All laboratories have to stay compliant with federal regulations. GL Technologies calibration services will always meet international standards, maintaining compliance with Good Laboratory Practices (GLP) and Good Manufacturing Practices (GMP). This not only will make sure of quality results but also creates trust among stakeholders and regulatory bodies.


With extensive experience in Shimadzu equipment, GL Technologies can save overall cost in the long run.  By minimizing downtime due to equipment failures and accurate results, laboratories avoid the repercussions of inaccurate analysis, including retesting, recalls, or compromised product quality.

Specialized Expertise

GL Technologies calibration services offer access to specialized expertise and advanced technological advancements. Our knowledgeable technicians possess in-depth understanding and training in Shimadzu HPLC systems, guaranteeing  calibrations to specific instrument models.

HPLC Calibration Education

Improve HPLC Performance Through Operational Qualification

HPLC (high-performance liquid chromatography) is a widely used analytical technique in the pharmaceutical and biopharmaceutical industries. HPLC provides researchers and analysts with reliable and quantitative data about the composition of complex mixtures. To verify accurate and reproducible results, it’s important to maintain and optimize the performance of the HPLC system. A vital step in this process is OQ (operational qualification). OQ can improve the performance of an HPLC system for organizations in a variety of ways.

Perfecting the Process: HPLC Calibration for Biopharmaceutical Analysis

High-performance liquid chromatography (HPLC) has become a critical tool in the biopharmaceutical industry for the analysis of various biological molecules, including proteins, peptides, nucleic acids, and other biopolymers. The accuracy and reliability of HPLC analysis are paramount to ensuring the quality, safety, and efficacy of biopharmaceutical products. This article provides a comprehensive overview of the importance of HPLC calibration for biopharmaceutical analysis and offers practical advice on optimizing the calibration process to achieve accurate results.

Fine-tuning HPLC Calibration for Biopharma Excellence

In the world of biopharmaceuticals, High-Performance Liquid Chromatography (HPLC) stands as a pillar analytical technique. It allows for the separation, identification, and quantification of compounds that are vital to the development, production, and quality control of biopharmaceuticals. HPLC calibration plays a role in maintaining the accuracy, reliability, and excellence of analytical results in this industry.

About GL Technologies

As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.

To speak with the experts about your Shimadzu HPLC needs, please fill out our online form or give us a call!



To request a quote from GL-Tec, please fill out our online form and we will get back to you promptly.


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