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HPLC Chromatography

HPLC Calibration: The Importance of Accurate Results

GL Techologies has a team of experts providing HPLC chromatography calibrations and certifications for biopharmaceutical and related industries for over 2 decades.  Companies of all sizes throughout California rely on the GL Technologies team for their HPLC equipment needs and requirements. 

High-performance liquid chromatography (HPLC) is a powerful analytical technique used in the biopharmaceutical industry to separate and analyze complex mixtures of biomolecules. It plays a crucial role in the development and production of biopharmaceuticals, which are large, complex molecules such as proteins, peptides, and nucleic acids that are produced using living cells.

To speak with the experts about your HPLC Calibration needs, please fill out our online form or give us a call!

858-202-1408

Trusted HPLC Chromatography Services


HPLC separates molecules based on their physicochemical properties such as size, charge, and hydrophobicity, using a stationary phase and a mobile phase. The stationary phase is typically a column packed with small beads or particles that are chemically modified to interact with the analyte of interest. The mobile phase is a solvent or mixture of solvents that carries the sample through the column.

One of the main challenges in biopharmaceutical analysis is the need for high sensitivity and resolution, as the samples can contain many similar or closely related molecules. HPLC addresses this challenge by providing high-resolution separation of complex mixtures, making it a powerful tool for quality control, process development, and formulation of biopharmaceuticals.

Different Types of HPLC Techniques in BioPharmaceutical


There are several different types of HPLC techniques used in the biopharmaceutical industry:

Exclusion chromatography (SEC)
Ion exchange chromatography (IEC)
Hydrophobic interaction chromatography (HIC)
Reversed-phase chromatography (RPC).

Each technique has its own advantages and is used for specific applications in biopharmaceutical analysis.

hplc chromatography process

Exclusion Chromatography (SEC)

Separates molecules based on their size, allowing for the separation of large biomolecules such as proteins and nucleic acids from smaller impurities.

Ion Exchange Chromatography

IEC separates molecules based on their charge, making it useful for separating proteins and other biomolecules that have different charge properties.

Hydrophobic Interaction Chromatography 

HIC separates molecules based on their hydrophobicity, while RPC separates molecules based on their polarity, making it useful for separating small molecules such as peptides and amino acids.

HPLC is used in many different stages of biopharmaceutical development and production, from early research and development to quality control and batch release. It is an essential tool for analyzing the purity, identity, and potency of biopharmaceuticals, and plays a critical role in ensuring the safety and efficacy of these life-saving therapies.

HPLC chromatography is an essential technique in the biopharmaceutical industry. It allows for the separation and analysis of complex mixtures of biomolecules, providing high-resolution separation of closely related molecules. HPLC is used in many different applications, including research and development, quality control, and batch release, and plays a critical role in ensuring the safety and efficacy of biopharmaceuticals.

hplc chromatography company

Benefits of HPLC Chromatography


High Performance Liquid Chromatography (HPLC) calibration is an essential process in ensuring accurate and reliable results in HPLC analysis. Here are four benefits of HPLC chromatography calibration:

Accurate quantification: Calibration ensures that the instrument is measuring the analytes accurately and precisely. It sets a reference point for the instrument response for a given concentration range, allowing for accurate quantification of the analyte(s) in the sample.

Reliable and consistent results: Calibration ensures that the instrument is functioning correctly, providing consistent and reliable results. It reduces the risk of errors and variability in the analysis, which could lead to incorrect conclusions and decisions.

Better sensitivity and resolution: Calibration can optimize the sensitivity and resolution of the instrument. By optimizing the instrument parameters, such as flow rate, column temperature, and detector sensitivity, calibration can improve the detection limit and the separation of closely related analytes.

Regulatory compliance: Calibration is required by regulatory agencies to ensure that the analytical results are accurate and reliable. By performing calibration regularly, the laboratory can demonstrate compliance with regulatory requirements, ensuring the validity of the results and maintaining the laboratory's accreditation.

To speak with the experts about your reverse phase HPLC Calibration needs, please fill out our online form or give us a call!

858-202-1408

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