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Irvine Agilent HPLC Calibration and Repair

HPLC is a technique used in the biopharma and pharmaceuticla industries for separating, identifying, and quantifying compounds with precision and accuracy. Among the leading providers of HPLC systems, Agilent Technologies has earned a reputation for excellence, offering cutting-edge instruments renowned for their reliability and performance.  GL Technologies has a deep understanding of Agilent HPLC systems when it comes to calibration and repair, helping our clients to maintain their equipment to the highest industry compliance and performance standards.

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!


Why Calibration and Repair Matter

Precision and Accuracy

Calibration validates that the instrument measures accurately by aligning it with standard reference materials, which guarantees the correct identification and quantification of compounds in a sample. Regular calibration prevents drift and ensures consistency in measurements over time. Repair, on the other hand, addresses any malfunctions or deviations from optimal performance, preserving the integrity of data output. In the realm of analytical chemistry, where minute differences can have significant implications, precise and accurate HPLC analysis is paramount for informed decision-making and advancing scientific understanding.


timely repair and maintenance of HPLC equipment prevent potential deviations in performance that could compromise data integrity, thereby safeguarding against regulatory non-compliance and the associated repercussions such as product recalls, fines, or even legal actions. Thus, diligent attention to HPLC calibration and repair not only upholds the credibility of analytical data but also serves as a cornerstone for maintaining adherence to regulatory requirements within the scientific community.

Maximizing Instrument Lifespan

Regular maintenance and repair not only prevent instrument downtime but also mitigate the risk of inaccuracies in analysis, which can have significant consequences in research, pharmaceutical, and industrial settings. Maximizing the lifespan of HPLC instruments through proper calibration and repair protocols not only safeguards investments but also maintains the integrity of experimental data, ensuring consistent and reliable outcomes over time.

Calibration Procedures for Agilent HPLC Systems

Performance Verification

Begin by confirming the proper functioning of the system's components, including pumps, detectors, and columns. Perform a system suitability test using standard reference materials to assess resolution, peak symmetry, and retention time consistency. Calibration of the flow rate should be carried out using a calibrated flow meter, adjusting as necessary to match the desired flow rate within acceptable tolerances. Detector wavelength accuracy and linearity should be verified using appropriate standards across the desired range. Finally, ensure proper integration parameters are set for accurate peak detection and quantification. Regular adherence to these calibration procedures guarantees the integrity of analytical results and maintains the HPLC system's performance at optimal levels.

System Suitability Testing

The process typically begins with verifying the system's performance using a standard reference material, checking parameters such as resolution, retention time, and peak symmetry. Calibration also involves assessing baseline noise, pressure, and flow rate stability. System suitability parameters are established based on method requirements and regulatory guidelines, with deviations triggering corrective actions or adjustments.

Calibration of Components

hen calibrating components of the system, meticulous attention must be paid to each element to maintain precision. Firstly, the column, the heart of the chromatographic separation, requires calibration for its efficiency, resolution, and retention time. This involves running standard mixtures and comparing the obtained data with reference values. Additionally, the pump's flow rate accuracy is essential and is typically verified using a calibrated flow meter or balance. Detector calibration involves adjusting the sensitivity, linearity, and wavelength accuracy through the use of standard solutions at different concentrations. Furthermore, the autosampler, responsible for injecting precise volumes of samples, necessitates calibration to ensure consistent and accurate sample introduction.

Repair and Maintenance Best Practices

Regular Inspections

Regular inspections are essential for maintaining the optimal performance of Agilent HPLC systems. Implementing best practices for repair and maintenance involves scheduling routine checks to identify potential issues before they escalate. During these inspections, it is imperative to meticulously examine critical components such as the pump, injector, detector, and column for signs of wear, leaks, or contamination. Additionally, verifying the integrity of seals, tubing, and connections ensures consistent and reliable chromatographic results. These proactive measures not only minimize downtime but also contribute to the longevity of the instrument, enhancing laboratory productivity and data quality.

Scheduled Maintenance

Scheduled maintenance is crucial for ensuring the optimal performance and longevity of Agilent HPLC systems. Implementing best practices for maintenance involves a systematic approach aimed at minimizing downtime and maximizing instrument reliability. Firstly, establishing a regular maintenance schedule based on manufacturer recommendations is paramount. This includes routine tasks such as inspecting and cleaning components, checking fluid levels, and verifying system pressures and temperatures. Additionally, proactive replacement of consumable parts like seals, filters, and tubing can prevent unexpected failures.

Professional Repairs

Professional repairs adhere to a set of best practices aimed at preserving instrument integrity and functionality. Firstly, diagnostics are conducted with precision to pinpoint underlying issues accurately. Technicians utilize genuine Agilent parts during repairs, guaranteeing compatibility and reliability.

More About Agilent Equipment

Agilent HPLC (High-Performance Liquid Chromatography) equipment stands as a hallmark of precision and reliability in analytical chemistry laboratories worldwide. Renowned for its advanced technological features and robust construction, Agilent's HPLC systems offer unparalleled performance in separating, identifying, and quantifying compounds in complex mixtures. Equipped with high-pressure pumps, precise autosamplers, sensitive detectors, and sophisticated data analysis software, Agilent HPLC instruments empower researchers to tackle a diverse array of analytical challenges with confidence and efficiency. Whether in pharmaceutical research, environmental analysis, or food safety testing, Agilent HPLC equipment consistently delivers accurate results, ensuring the integrity and quality of scientific investigations.

Beyond its technical prowess, Agilent HPLC equipment distinguishes itself through its user-friendly interface and intuitive operation. Designed with the needs of both seasoned chromatographers and novice users in mind, Agilent systems facilitate seamless method development, optimization, and execution. The platform's modularity allows for customization to suit specific application requirements, while its robustness ensures long-term reliability and minimal downtime. With a commitment to innovation and customer satisfaction, Agilent continues to lead the industry in HPLC technology, empowering scientists to push the boundaries of analytical chemistry and accelerate discoveries that benefit society.

The Calibration Corner

HPLC Calibration: Accuracy in Analytical Chemistry

Analytical chemistry plays an important role in various scientific fields, from pharmaceuticals to environmental monitoring, where precision and accuracy are paramount. High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, allowing for the separation, identification, and quantification of components in complex mixtures. The reliability of HPLC results heavily relies on the proper calibration of equipment and attention to detail. Calibration is a process that verifies the accuracy, precision, and reliability of the HPLC system, protecting against errors that could compromise data integrity. Let's look at the significance of HPLC calibration, its key components, and the methods used to maintain accuracy in analytical chemistry.

Fine-tuning HPLC Calibration for Biopharma Excellence

In the evolving world of biopharmaceuticals, High-Performance Liquid Chromatography (HPLC) stands as a pillar analytical technique. It allows for the separation, identification, and quantification of compounds that are vital to the development, production, and quality control of biopharmaceuticals. HPLC calibration plays a role in maintaining the accuracy, reliability, and excellence of analytical results in this industry.

GL Technologies Overview

As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.  We serve biopharma and related companies throughout Orange County including Anaheim and Long Beach. If you are in need of fume hood certification for your lab, we can help as well!

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!



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