VOSB
Service/Sales/ General Inquiries 858-202-1408

Long Beach HPLC Calibration and Repair

Biopharma and pharmaceutical organizations throughout Long Beach rely on GL Technologies for HPLC calibration and repair services. Our goal is to verify the accuracy and reliability of HPLC systems to maintain the quality and safety of pharmaceutical products. Calibration and timely repair of HPLC equipment play importantl roles in this process, protecting against inaccuracies that could compromise product potency and regulatory compliance. The team at GL Technologies has extensive experience working with biopharma and related industries with HPLC calibration, HPLC repairs, and HPLC maintenance services.

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!

858-202-1408

HPLC Calibration Explained


HPLC calibration is the systematic process of verifying and adjusting the performance of chromatographic equipment to maintain accurate and consistent results. Calibration involves evaluating various parameters including flow rate, pressure, temperature, detector sensitivity, and wavelength accuracy. By calibrating HPLC instruments regularly, pharmaceutical companies can meet strict quality standards and comply with regulatory requirements.

Calibration in Pharmaceutical Analysis


In pharmaceutical analysis, precise and reliable measurements are invaluable for product formulation, stability testing, impurity profiling, and dissolution studies. Properly calibrated HPLC systems allow scientists to accurately quantify active pharmaceutical ingredients (APIs) and impurities, which maintains product potency, safety, and compliance with regulatory standards such as Good Manufacturing Practices (GMP).

GL-Tec Steps in HPLC Calibration


Performance Verification


The GL Technoogies team starts by makin sure that the HPLC system operates accurately, maintaining the integrity of analytical results. Performance verification involves evaluating key parameters including retention time, peak symmetry, resolution, and system suitability, often through the use of standard reference materials. By confirming the instrument's performance against predefined criteria, analysts and your team can confidently rely on the HPLC system for reproducible measurements.

Standard Preparation


Standard preparation involves accurately measuring a known quantity of the analyte of interest and dissolving it in a suitable solvent to achieve a predetermined concentration. This process requires precision to for the accuracy of the calibration curve. Factors such as the purity of the standard compound, the choice of solvent, and the stability of the solution will be carefully considered to prevent inaccuracies in subsequent analyses.

System Suitability Testing


System suitability testing involves monitoring key parameters such as resolution, peak symmetry, retention time, and peak area reproducibility, typically using a standard mixture of compounds. By conducting system suitability testing before sample analysis, analysts can verify the performance of the HPLC system, identify any potential issues, and take corrective actions if necessary, which maintains ensuring the accuracy of analytical results.

Calibration Adjustments


Calibration adjustments involve fine-tuning various guidelines including flow rate, column temperature, detector sensitivity, and mobile phase composition to match the instrument's performance with established standards. This process requires careful attention to detail and often involves adjustments to achieve peak separation and peak resolution. By calibrating the HPLC system, scientists can increase the validity of their analytical measurements, which validates the integrity of analytical data.

Documentation


GL Technologies provides comprehensive documentation recording of the instrument's configuration, including areas such as column dimensions, detector settings, and mobile phase composition. Precise calibration procedures, including system suitability tests and standard solution preparations will be methodically documented to establish a strong analytical framework. Any deviations from established protocols, corrective actions, and instrument maintenance activities will be diligently recorded to facilitate comprehensive analysis and troubleshooting.

Common Challenges in HPLC Calibration


Despite its importance, HPLC calibration can present challenges including instrument drift, baseline instability, and variability in peak retention. Factors like solvent quality, column degradation, and detector aging can also affect calibration accuracy. To minimize these challenges, it's important to have strong calibration protocols, use high-quality standards, and perform regular maintenance checks on HPLC components.

HPLC Repair and Maintenance


In addition to calibration, proactive maintenance and timely repair are vital for HPLC performance and minimizing downtime. The GL Technologies routine maintenance tasks include cleaning, replacing worn-out components, and troubleshooting instrument malfunctions. For complex repairs or troubleshooting issues, our team is here and ready to help!

Popular Articles


HPLC Calibration: Accuracy in Analytical Chemistry

Analytical chemistry plays an important role in various scientific fields, from pharmaceuticals to environmental monitoring, where precision and accuracy are paramount. High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, allowing for the separation, identification, and quantification of components in complex mixtures. The reliability of HPLC results heavily relies on the proper calibration of equipment and attention to detail. Calibration is a process that verifies the accuracy, precision, and reliability of the HPLC system, protecting against errors that could compromise data integrity. Let's look at the significance of HPLC calibration, its key components, and the methods used to maintain accuracy in analytical chemistry.

Fine-tuning HPLC Calibration for Biopharma Excellence

In the evolving world of biopharmaceuticals, High-Performance Liquid Chromatography (HPLC) stands as a pillar analytical technique. It allows for the separation, identification, and quantification of compounds that are vital to the development, production, and quality control of biopharmaceuticals. HPLC calibration plays a role in maintaining the accuracy, reliability, and excellence of analytical results in this industry.

GL Technologies


As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.  We serve biopharma and related companies throughout Orange County including Anaheim and Irvine.

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!

858-202-1408

REQUEST A QUOTE

To request a quote from GL-Tec, please fill out our online form and we will get back to you promptly.

CLICK HERE TO BEGIN