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Irvine HPLC Calibration and Repair Services

Biopharma and pharmaceutical organizations throughout Irvine rely on HPLC equipment to maintain the quality and potency of their products.  To harness an HPLC systems full potential, organizaitons need to prioritize proper calibration, maintenance, and repair of their HPLC systems. The team at GL Technologies has decades of experience in HPLC calibration, HPLC repairs,a nd HPLC maintenancne programs so companies throughout the Irvine area can maintain accuracy of their products and meet regulatory standards.

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!

858-202-1408

HPLC Calibration Overview


Calibration is the key to reliable HPLC performance. It involves verifying and adjusting the instrument to provide accurate and reproducible results. For biopharma companies, meeting regulatory standards such as Good Manufacturing Practices (GMP) is non-negotiable. Calibration should include a review of the equipments flow rate, detector sensitivity, retention time, and column efficiency. Establishing a strong calibration protocol tailored to the specific requirements of biopharmaceutical analysis is vital for maintaining compliance and product quality.  We service all manufacturer makes and models including Agilent, Shimadzu, and more.

GL Tec Steps in HPLC Maintenance


Regular maintenance is required for preserving the integrity and longevity of HPLC systems. Biopharma and pharmaceutical oganizations should implement a proactive maintenance schedule to prevent downtime and maintain consistent performance. GL Technologies routine tasks include:

Cleaning and Flushing


Cleaning and flushing are the first steps in our maintenance routine. To start, we provide thorough cleaning of the system components, including the injector, column, and detector, to remove any residual sample or contaminants that may accumulate over time, potentially affecting chromatographic performance. This involves flushing the system with solvents of increasing polarity to effectively remove all traces of compounds. Along with this, flushing the system with a compatible mobile phase helps to eliminate any residual solvents or buffer salts that may remain, making sure the system is ready for the next analysis. Regular cleaning and flushing not only prolong the lifespan of the HPLC system but also contribute to the accuracy of analytical results.

Column Care


It's imperative to monitor column pressure regularly and replace inlet and outlet frits as needed to prevent backpressure buildup and maintain peak chromatographic performance. Storing the column in a protective environment, such as a guard column or proper storage solution, can protect it from damage and increase its lifespan.

System Checks


Our HPLC system checks involve inspecting critical components including the injector, detector, pump, and columns for any signs of wear, contamination, or malfunction. We will verify proper fluidic connections, check for leaks, and confirm that the system pressure and flow rates are within the specified ranges. Performing routine system checks not only helps prevent unexpected downtime but also verifies the reproducibility of chromatographic analysis, which contributes to the effectiveness of your HPLC system.

Software Updates


Software updates often include bug fixes, performance improvements, and compatibility improvements that address potential issues and streamline operations. By staying current with software updates provided by the manufacturer, HPLC operatores can minimize the risk of system errors and maximize instrument longevity. Updated software may offer new features or functionalities that improve analytical capabilities, which supports efficient laboratory workflows and scientific research goals. Our team will integrating software updates into the routine maintenance schedule to maintain the reliability of your organizations HPLC systems.

Effective HPLC Repair Strategies


Despite HPLC maintenance efforts, HPLC systems may encounter issues that require repair. Biopharma companies should have contingency plans in place to minimize disruptions and quickly address instrument malfunctions. GL Technologies works with biopharmaceutical and pharmaceutical organizations for routine maintenance on their HPLC systems to keep them working properly.

Troubleshooting Protocols


If your HPLC system does need repairs, our first step involves a thorough inspection of the system, focusing on components such as the pump, injector, column, and detector. By evaluating each element for signs of damage or irregularities, our technicians can pinpoint the root cause of the issue. We use diagnostic tools such as pressure gauges and test injections aids in the identification of problems. Subsequently, targeted interventions, ranging from component replacements to adjustments in operating parameters, are used to fix the issue and restore top performance.

Partnering with Experts


Partnering with the skilled technicians at GL Technologies provides swift diagnosis, troubleshooting, and reliable solutions tailored to the specific needs of the instrument. By trusting the repairs to the professionals at GL Tec who are well-versed in HPLC intricacies, laboratories can minimize downtime, maintain performance, and protect the integrity of their analytical workflows. Our partnerships create proactive maintenance practices,allowing laboratories to preemptively address potential issues and extend the longevity of their HPLC equipment.

Spare Parts Management


Central to our approach is maintaining a comprehensive inventory of critical components, from columns and seals to detectors and pumps. Regular audits to evaluate inventory levels against usage patterns and anticipated maintenance needs enable proactive restocking, averting unexpected disruptions. Which is why we also implement CMMS Software for parts management.

Training and Skill Development


Laboratory technicians must undergo training to understand the intricate workings of HPLC instruments, including troubleshooting common issues and performing routine maintenance tasks. GL Technologies training will include theoretical knowledge of chromatography principles as well as hands-on experience with various HPLC models.

Articles


HPLC Calibration: Accuracy in Analytical Chemistry

Analytical chemistry plays an important role in various scientific fields, from pharmaceuticals to environmental monitoring, where precision and accuracy are paramount. High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, allowing for the separation, identification, and quantification of components in complex mixtures. The reliability of HPLC results heavily relies on the proper calibration of equipment and attention to detail. Calibration is a process that verifies the accuracy, precision, and reliability of the HPLC system, protecting against errors that could compromise data integrity. Let's look at the significance of HPLC calibration, its key components, and the methods used to maintain accuracy in analytical chemistry.

Fine-tuning HPLC Calibration for Biopharma Excellence

In the evolving world of biopharmaceuticals, High-Performance Liquid Chromatography (HPLC) stands as a pillar analytical technique. It allows for the separation, identification, and quantification of compounds that are vital to the development, production, and quality control of biopharmaceuticals. HPLC calibration plays a role in maintaining the accuracy, reliability, and excellence of analytical results in this industry.

GL Technologies


As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.  We serve biopharma and related companies throughout Orange County including Anaheim and Long Beach. If you are in need of fume hood certification for your lab, we can help as well!

To speak with the experts about your HPLC needs, please fill out our online form or give us a call!

858-202-1408

REQUEST A QUOTE

To request a quote from GL-Tec, please fill out our online form and we will get back to you promptly.

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