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Riverside Mass Spec Calibration

For biopharmaceutical and pharmaceutical organizations through the Riverside area, mass spectrometry stands as a key technology for identifying and quantifying molecules within a sample. To maintain accuracy and reliability in mass spectrometric analysis, calibration is as an indispensable process. The experts at GL Technologies have extensive experience in mass spec calibration and certification, we will come to your location to perform the proper testing so your mass spec equipment works at peak performance.

To speak with the experts about your mass spec calibration needs, please fill out our online form or give us a call!


What is Mass Spectrometry Calibration?

Mass spectrometry calibration refers to the process of tuning and validating the accuracy of mass spectrometers. This calibration involves adjusting the instrument's settings and parameters to accurately measure the mass-to-charge ratio (m/z) of ions produced from a sample. Calibration compensates for instrumental variations, verifying that the detected masses align correctly with the known masses of reference compounds.

Importance of Calibration in Mass Spectrometry


This calibration process establishes a correlation between the detected signals and the actual masses of compounds, allowing for the accurate identification and quantification of substances within a sample. Accurate calibration not only increases the instrument's performance but also validates the validity of scientific findings, maintaining reproducibility and consistency in experimental outcomes. By calibrating mass spectrometers, scientists and researchers can confidently unravel complex molecular structures, chemical compositions, and conduct exact quantitative analyses across biopharma and pharmaceutical industries.

Sensitivity Improvement

Precision is vital in research and pharmaceuticals where the smallest quantities of substances must be detected. Sensitivity improvement through calibration increases the instrument's performance, refining its ability to detect and differentiate compounds even at trace levels, which expands the scope of analysis and allows for the discovery of compounds that might otherwise remain undetected.

Quality Control

This mass spec calibration process involves the adjustment of instruments to align with known reference standards, allowing for the determination of unknown sample constituents with accuracy. In the world of quality control, calibration serves as the pin for establishing traceability, validating analytical methods, and verifying the consistency and reliability of results. It not only protects against errors and inaccuracies but also makes sure that the instruments are operating at peak capacity within defined parameters, which guarantees the integrity of data generated.


By calibrating mass spec instruments regularly, scientists and researchers can validate the accuracy of measurements, detect any potential deviations or drifts in instrument performance, and maintain reproducibility across analysis. The calibration not only improves the confidence in reported data but also validates the comparability and consistency of results, which maintains the reliability and credibility of scientific findings in fields like pharmaceuticals and biomolecular research.

Methods of Mass Spectrometry Calibration

External Calibration

External calibration is a fundamental method in mass spectrometry used to accurately determine the mass-to-charge ratios (m/z) of ions within a sample. This calibration technique involves introducing known standard compounds of measured masses into the mass spectrometer before the analysis of the sample of interest. These standards, referred to as calibrants, cover a range of masses and are chosen based on their stability, purity, and compatibility with the instrument. By analyzing the spectra produced by the calibrants and comparing their observed m/z values with their known theoretical masses, a calibration curve or calibration equation can be generated. This calibration curve is then used to convert the observed m/z values of the sample ions into accurate mass measurements, allowing for the identification and quantification of compounds within the sample with high precision and reliability.

Internal Calibration

Internal calibration in mass spectrometry refers to a method used to calibrate and improve the accuracy of mass measurements within the instrument itself, without the need for external calibration standards. This technique involves introducing reference compounds or ions of known mass into the sample prior to or during the analysis process. These reference masses serve as calibration markers, allowing the mass spectrometer to adjust its measurements based on the known values of these internal standards. By comparing the detected mass-to-charge ratios of the sample ions with those of the internal standards, the instrument can correct for any deviations or inaccuracies, which improves the precision and reliability of mass measurements.

Mass Calibration Software

This calibration process involves the use of reference compounds with known mass values, which are analyzed alongside unknown samples. The software uses these reference compounds to generate calibration curves, establishing the relationship between the mass-to-charge ratios (m/z) and the actual masses. Through adjustments, the software fine-tunes parameters such as voltage, resolution, and sensitivity to align the detected m/z values with the known masses of reference compounds.

Challenges and Considerations

Instrument Stability

Variations in environmental conditions, such as temperature or humidity, can impact instrument stability, necessitating regular recalibration.

Choice of Standards

Selecting appropriate reference compounds or standards is critical to ensure accurate calibration across a broad range of analytes and masses.

Routine Maintenance

Regular maintenance and calibration checks are crucial to sustain the accuracy and performance of mass spectrometers over time.

Mass Spec Education

Mass Spectrometry: Unlocking Biopharmaceutical Insights

In the world of biopharmaceuticals, understanding the intricate details of molecular structures is of the utmost importance. This comprehension is vital not only for drug discovery and development but also for maintaining the safety, efficiency, and quality of pharmaceutical products. Among the various analytical techniques available, mass spectrometry stands out as a powerful tool that plays an important role in unraveling the mysteries of biopharmaceuticals.

A Deep Dive into Mass Spectrometry Techniques

Mass spectrometry (MS) has emerged as a powerful analytical technique revolutionizing various scientific fields, particularly in biopharma. Its ability to precisely determine the mass-to-charge ratio of ions makes it indispensable in drug development, biomarker discovery, proteomics, and more. This comprehensive guide aims to explore the diverse applications, principles, and advancements in mass spectrometry techniques.

GL Technologies Capabilities

As a full-service company specializing in equipment calibration, repair, and certification services for biopharmaceutical, pharmaceutical, and medical device industries. Our team has extensive experience working with sPRT calibrations along with CMMS softwareHPLC OQ validation, and fume hood certifications. Companies of all sizes rely on our team to implement, maintain, and keep their research and manufacturing processes compliant with regulatory standards. Other specialties include building maintenance systems, and mass spectrometry calibrations.  Our team works with clients throughout San Diego, Los Angeles, and San Francisco covering the entire State of California with our services!

To speak with the experts about your mass spec calibration needs, please fill out our online form or give us a call!



To request a quote from GL-Tec, please fill out our online form and we will get back to you promptly.


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